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Upload organization:University of Tsukuba Graduate School of Life and Environmental Sciences  Upload date:2011/10/18

Crosstalk regulation between the formation of the transcriptional environment and amino acid metabolism

Professor Akiyoshi Fukamizu
Graduate School of Life and Environmental Sciences
University of Tsukuba
http://tmsystem.tara.tsukuba.ac.jp/english/



Summary
Methylation of DNA and lysine and arginine residues is a major determinant of the formation of the transcriptional environment. The main methyl donor, S-adenosyl-L-methionine (SAM), is synthesized from methionine and ATP by SAM synthetase (SAMS) or methionine adenosyltransferase (MAT). This suggests a close linkage between the formation of the transcriptional environment and amino acid metabolism. However, little is known about the crosstalk regulation between these biological processes.

This project seeks to understand the fundamental importance of methyl transfer within the transcriptional environment. In order to unveil the biochemistry and genetics of basic biological functions, the interactions between methyl transfer and SAM metabolic pathways will be investigated in four types of nematode (C. elegans) SAMS mutants (sams-1, sams-3, sams-4 and sams-5). There are 116 methylase genes identified in nematodes to date, encoded in the genomes at a proportion of approximately 1% of all protein-coding genes. More specifically, our genetic and biochemical analyses will employ RNA interference knockout techniques to identify the methyltransferases directly involved in the e SAMS functions. In cooperation with our coinvestigator (H.T.), we will study the metabolic pathways of other amino acids than methionine in a mouse model, and explore the crosstalk regulation between these pathways and the formation of the transcriptional environment.